These charts are from the Canadian study on children and transmission. They are from the supplemental material and show the distribution of cycle numbers and relationship to virus viability; that is could virus be cultured. You can see that when you get to 30 cycles, there is basically zero likelihood that a person is actually infected. And really, the likelihood of infectiousness is pretty low starting by 21 or 22 cycles. The first chart shows pediatric patients, the second shows adults. It is very striking how quickly accuracy falls off as cycle number rises. Just a reminder, sensitivity basically tells you how many false negatives to expect, so if sensitivity is 98%, you would expect 2% false negatives. Specificity tells you how many false positives to expect, so if specificity is 95%, you would expect 5% false positives. That is ignoring true prevalence in the population, but that probably doesn’t matter for this study. Now careful with study, read the label, this is likelihood of culture negativity, so you kind of have to invert your thinking, So where you see those 100% specificity numbers, there is no chance that the culture was not in fact negative, i.e. the person wasn’t actually infected.